Cultural studies of pigeonpea, Mycovellosiella cajani (Henn.)

  • E W Kimani
  • A W Mwang'ombe
  • P M Kimani

Abstract

Experiments were carried out to determine a culture medium, temperature and light regimes conducive for mycelial growth and sporulation of Mycovellosiella cajani obtained at Kabete field station and found to be highly pathogenic to pigeonpea plants. The results showed that this isolate of M. cajani had a more fastidious nutritional requirement for growth than for sporulation. Selective sub-culturing produced colonies that had higher sporulation. The M. cajani test isolate showed moderate sporulation in carrot leaf decoction agar and potato dextrose agar. The lowest sporulation was noted in potato carrot agar, carrot agar, and pigeonpea meal agar.  bundant sporulation was obtained after 10 days in pigeonpea leaf decoction agar incubated at room temperature (20-24°C) in a 24 h light regime. A conidial concentration of 2 x 104 conidia per millilitre gave the highest sporulation in pigeonpea leaf decoction agar after 14 days of incubation. Colony growth and sporulation occurred at 15, 20 and 25°C with optimum at 20°C. Colony growth did not occur at 5, 10, 30 and 35°C. Sporulation occurred under all the environmental factors which supported growth.

Published
2008-09-08
How to Cite
Kimani, E., Mwang’ombe, A., & Kimani, P. (2008). Cultural studies of pigeonpea, Mycovellosiella cajani (Henn.). East African Agricultural and Forestry Journal, 67(1&2). Retrieved from https://www.kalro.org/www.eaafj.or.ke/index.php/path/article/view/63