In-silico epitope prediction of small ruminant morbillivirus proteins as potential vaccine candidates

  • W Adero Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology
  • S Nyanjom Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology
  • M. K. Mwirigi Kenya Agricultural and Livestock Research Organisation, Biotechnology Centre Kabete,
  • B. Yattinder Kenya Agricultural and Livestock Research Organisation, Biotechnology Centre Kabete,

Abstract

Small ruminant morbillivirus or Peste des petits ruminants (PPR) is an acute and highly contagious viral disease affecting ruminants, especially sheep (Ovis aries) and goats (Capra aegagrus), with goats being more adversely affected. In silico techniques offer a promising and cost-effective approach for identifying potential epitopes as a vaccine candidate. The objective of the
studywas to predict effective multi-epitope candidates against Peste des petits ruminants’ virus (PPRV) from two immunogenic proteins Haemagglutinin (H), and Fusion (F). An immuno-informatics approach was used to design a multi-epitope subunit vaccine peptide consisting of linear B- and T-cell epitopes that reported potential novel vaccine candidates. Multiple sequence alignment (MSA) as done using MEGA 10.2.4., Immune Epitope Database (IEDB) resource was then used to select the conserved B and T cell epitopes. Potential antigens were then selected using VaxiJen v2.0 server at a threshold value of 0.4. Conservancy and homology modelling was also predicted, and allergenicity was anticipated through AllerTOP v. 2.0 with 88.7% accuracy. The toxicity of the peptides was predicted using the Toxin Pred server. Finally, homology modelling of the 3D structures of the vaccine candidate for PPRV was performed using the raptorX server and visualized using PyMOL molecular graphics system version 4.0. Conservation of the proteins and predicted epitopes suggested that the generated chimera could be used for cross-protection. The predicted epitopes offered a promising vaccine candidate for the control and management of PPR in small ruminants.

Published
2022-05-25
How to Cite
Adero, W., Nyanjom, S., Mwirigi, M. K., & Yattinder, B. (2022). In-silico epitope prediction of small ruminant morbillivirus proteins as potential vaccine candidates. East African Agricultural and Forestry Journal, 85(1-4), 14. Retrieved from https://www.kalro.org/www.eaafj.or.ke/index.php/path/article/view/489