Assessment of infection and/or contamination of rosecoco GLP-2

  • A W Mwang'ombe
  • S O Otieno
  • M Shankir

Abstract

Detection of contaminant and infectious Pseudomonas syringae pv. phaseolicola was carried out using the dilution plate method in bean seed samples collected from farmers in Kisii, Nyeri and Meru Districts, Kenya. Certified commercial and diseasefree bean samples were included in the study. The confirmatory tests of the pathogen identity used in the experiments were fluorescein production, oxidase activity, arginine dihydrolase activity, levan production, gelatine liquefaction, nitrate reductase activity, phaseolotoxin production, bioassay and pathogenicity. Subsequently, determination of bean seeds was carried out using bean seed samples from small-scale farmers and the certified commercial bean seed sample. The estimated infection levels were determined by applying the most probable number method to the results of the dilution planting assays. Only 14% of the bean seed samples were free of the halo blight pathogen both on the surface and inside. Of these, half had the pathogen on the surface only and the rest had both internal and contaminant infections by P. syringae pv. phaseolicola. The bacterium was detected as an internal infectant only in certified commercial bean seeds. Subsequently, determination of the level of surface and internal infection of bean seeds was ca rried out using bean seeds. The infection levels in farmers' seed varied from 0.1 to 1.1% as compared to 0.3% for certified commercial seeds. The ratio of the level of seed infection to the subsequent level of seedling infection was determined in a greenhouse study and found to be about 1:1.

Published
2008-10-14
How to Cite
Mwang’ombe, A., Otieno, S., & Shankir, M. (2008). Assessment of infection and/or contamination of rosecoco GLP-2. East African Agricultural and Forestry Journal, 60(1). Retrieved from https://www.kalro.org/www.eaafj.or.ke/index.php/path/article/view/210