A study was conducted to determine whether trypanosomes dissemination after being deposited by tsetse was by direct entry into the blood or by migration through the lymphatic system. An approach which enabled continuos monitoring of lymph flow from lymph nodes draining sites of development of local skin reactions induced by cyclically transmitted (T. congolense) in cattle was undertaken. From the results, it was concluded that (T. Congolense) was disseminated from the site of localised proliferation in the skin to the bloodstream through the drainage lymphatic system. Cell mediated immune responses were detected 3 days after cyclical infection. Specific humoral responses were detected two weeks after infection of animals. Results further showed that the skin and drainage nodes were important in inducing protective immunity.